Electron spin resonance and spectrophotometry investigation of singlet oxygen formation during tetracycline autoxidation

✍ Scribed by T. Michalska; K. Lichszteld; I. Kruk; S. Marczyński

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 482 KB
• Volume: 19
• Category: Article
• ISSN: 1011-1344
• DOI: 10.1016/1011-1344(93)80093-o

✦ Synopsis

The generation of 'Oa '4 during autoxidation of tetracychnes (tetracycline, oxytetracycline, chlortetracycline and methacycline) in the presence of Co(R) ions was studied using electron spin resonance (ESR) and spectrophotometry methods. 2,2,6,6_tetramethylpiperidine was used as a trap in the ESR study. The spectrophotometry determination of 'Oa was based on bleaching of p-nitrosodimethylaniline (RNO) caused by the product of the reaction of '02 with imidazole and was followed by monitoring the decrease in optical density at 440 nm. The effect of 'Oa quenchers on the concentration of nitroxide radicals and on the bleaching of RN0 was studied.