Electron microscopy of ribosomal RNA mounted by adsorption in the absence of protein

✍ Scribed by Thomas R. Johnson; J. Douglas Caston

• Book ID: 102763746
• Publisher: Wiley (John Wiley & Sons)
• Year: 1975
• Tongue: English
• Weight: 749 KB
• Volume: 14
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1975.360140715

✦ Synopsis

Abstract

A method is described for visualizing ribosomal RNA in the electron microscope by an adsorption technique. The visual configuration of the molecules is sensitive to the composition of the medium from which they are adsorbed, and is reproducible under a given set of conditions. Regular structural features occur in the larger rRNA species similar to those observed with protein monolayer techniques. The dimensions of adsorbed molecules, however, are more consistent with current models of ribosomal RNA as it exists in solution.