Electron Microscopy Methods and Protocols Volume 117 || High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling
โ Scribed by Hajibagheri, M. A. Nasser
- Book ID
- 120308468
- Publisher
- Humana Press
- Year
- 1999
- Weight
- 298 KB
- Category
- Article
- ISBN-13
- 9781592592012
No coin nor oath required. For personal study only.
โฆ Synopsis
Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.
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