Electron microscopic observations on the process of polar body formation in artificially activated eggs of the rose bitterling

✍ Scribed by Ohta, Tadayuki

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 949 KB
• Volume: 237
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402370212

✦ Synopsis

Mature eggs were obtained from adult female rose bitterling, Rhodeus ocellatus ocellatus. Both intact and dechorionated eggs were activated by immersion in freshwater. The formation of the second polar body after egg activation was examined ultrastructurally by both scanning and transmission electron microscopy. The second polar body was extruded from a specialized site identified by an aggregation of thick leaf-like projections of the egg cytoplasm, a short distance from the sperm entry site about 15 min postactivation. After extrusion, formation of a cleavage furrow was completed at the base of the polar body protuberance about 30 min postactivation. The nucleus of the extruded second polar body had a nuclear envelope. The formation of the second polar body in activated eggs are discussed.