Electron microscopic investigations on the structure of lectin I from Viscum album L.
✍ Scribed by G. Lutsch; F. Noll; P. Ziska; A. Kindt; H. Franz
- Book ID
- 115916326
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 599 KB
- Volume
- 170
- Category
- Article
- ISSN
- 0014-5793
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Two glycopeptide fractions prepared from mistletoe (Viscum album) lectin I by Pronase digestion were fractioned by affinity chromatography on a concanavalin A-Sepharose column. With 400-MHz 1H NMR spectroscopy, in conjunction with sugar analysis, the following oligosaccharide structures could be det
## Abstract The complete primary structure of the A chain of mistletoe lectin III (ML3A), a type II ribosome‐inactivating protein, was determined using proteolytic digests of ML3A, HPLC separation of the peptides, Edman degration and MALDI‐MS. Based on our results, ML3A consists of 254 amino acid r