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Electron capture dissociation of substance P using a commercially available Fourier transform ion cyclotron resonance mass spectrometer

✍ Scribed by Jan Axelsson; Magnus Palmblad; Kristina Håkansson; Per Håkansson

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
65 KB
Volume
13
Category
Article
ISSN
0951-4198

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✦ Synopsis

Electron capture dissociation of the peptide Substance P is reported for the first time, with an unmodified, commercially available Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The fragmentation pattern is compared with that obtained with collisionally induced dissociation of the ions in the electrospray ion source, and note that electron capture dissociation gives a more easily interpreted spectrum, showing mainly C-fragments. With the exception of the proline residues, which require cleavage of two chemical bonds, we observe all C-fragmental we find the bias voltage of the electron gun not to be very critical.

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✍ Steven A. Hofstadler; Richard H. Griffey; Ljiljana Pasa-Tolic; Richard D. Smith 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 85 KB 👁 2 views

In this work we demonstrate the use of peptide as a stable internal mass standard to obtain accurate mass measurements of infrared multiphoton dissociation (IRMPD) generated phosphorothioate oligonucleotide fragment ions. Because oligonucleotides are more readily dissociated in the gas phase by IRMP