Electromagnetic fields enhance the stress response at elevated temperatures in the nematode Caenorhabditis elegans
✍ Scribed by Bernd Junkersdorf; Hartmut Bauer; Herwig O. Gutzeit
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 131 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0197-8462
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✦ Synopsis
We have studied the effect of extremely low frequency electromagnetic fields (ELF-EMF) in the presence of a second stressor (mild heat shock) on the expression of a lacZ reporter gene under the control of hsp16 or hsp70 promoters in two transgenic strains of C. elegans. The expression of the reporter gene was studied by scoring animals with induced b-galactosidase activity after staining in toto or by biochemical quantitation of the enzyme activity, respectively. In our experimental setup we were able to expose the animals to 50 Hz magnetic flux density of 0±150 mT and at the same time control temperature with high precision ( AE 0.1 C). Experimental conditions were defined for which EMF strongly enhances the expression of the reporter gene. Bioelectromagnetics 21:100±106, 2000.