Electrokinetic injection of DNA from gel micropads: Basis for coupling polony technology with CE separation
✍ Scribed by Olga Kosobokova; Dmitri N. Gavrilov; Vyacheslav Khozikov; Andrey Stepukhovich; Andriy Tsupryk; Sergey Pan'kov; Olga Somova; Nikolai Abanshin; Georgiy Gudkov; Marina Tcherevishnik; Vera Gorfinkel
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 870 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Abstract
We propose a novel method for electrokinetic injection of DNA samples into capillaries from nanoliter gel micropads, deposited on glass slides, which are coated with electroconducting film. Theoretical and experimental proof is presented for the proposed method. The method allows efficient and highly precise injection without physical contact between the gel pad and the capillary. Read length of more than 700 bp at Q20 has been reproducibly demonstrated in fused‐silica capillaries using the proposed injection technique. Based on the obtained results we discuss a novel DNA sequencing system which combines DNA amplification and cycle sequencing in arrays of subnanoliter gel micropads and high‐throughput electrophoretic separation in monolith multicapillary arrays.