Electrofusion of Penicillium protoplasts after dielectrophoresis

✍ Scribed by Dr. I. Groth; H.-E. Jacob; W. Künkel; H. Berg

• Book ID: 102389907
• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 278 KB
• Volume: 27
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620270614

✦ Synopsis

The protoplast fusion of auxotrophic Penicillium mutats after dielectrophoretic cell contact and rectangular field pulses (4,2 kV/cm, 25 ps) was microscopically observed in a microchamber consisting of two vapour-plated chromium electrodes on a microslide (gap between the electrodes of about 150 pm). The conditions for protoplasting of Penicillium and for electrofusion are described. Isolation of viable fusion products was realized.