Electrochemiluminescence DNA Sensor Based on Hairpin Structure DNA as recognition element and Ru(bpy)32+-doped silica Nanoparticles as Signal-Producing Compound

✍ Scribed by Qixia Sun; Guizheng Zou; Xiaoli Zhang

• Book ID: 102179139
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 389 KB
• Volume: 23
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.201100331

✦ Synopsis

Abstract

An electrochemiluminescence (ECL) DNA sensor was presented using a hairpin structure DNA as recognition element and Ru(bpy)~3~^2+^‐doped silica nanoparticles (Ru‐DSNPs) as ECL signal‐producing compound. The sensor was fabricated by immobilizing a hairpin structure DNA probe labeled with Ru‐DSNPs onto a gold electrode and formimg the stem‐loop structure. This sensor generated a strong ECL. After hybridization with the target DNA, the ECL signal significantly decreased. The logarithm of ECL intensity decreased linearly with the logarithm of target DNA concentration in the range from 2.0×10^−13^ to 2.0×10^−9^ mol/L with detection limit was 5.0×10^−14^ mol/L (S/N=3).