Electrochemical study in both classical cell and microreactors of flavin adenine dinucleotide as a redox mediator for NADH regeneration
✍ Scribed by Theodore Tzedakis; Kane Cheikhou; Roche Jerôme; Groenen Serrano Karine; Reynes Olivier
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 686 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0013-4686
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✦ Synopsis
The electrochemical reduction of flavin adenine dinucleotide (FAD) is studied in a classical electrochemical cell as well as in two types of microreactors: the first one is a one-channel reactor and the other one, a multichannel filter-press reactor. The ultimate goal is to use the reduced form of flavin (FADH 2 ), in the presence of formate dehydrogenase (FDH), in order to continuously regenerate the reduced form of nicotinamide adenine dinucleotide (NADH) for chiral syntheses. Various voltammetric and adsorption measurements were carried out for a better understanding of the redox behavior of the FAD as well as its adsorption on gold. Diffusivity and kinetic electrochemical parameters of FAD were determined.