Electrochemical Methods for the Investigation of Bioaffinity Interactions Based on Gold Nanoparticles Modified Sensors

✍ Scribed by Victoria Shumyantseva; Elena Suprun; Tatiana Bulko; Alexander Archakov

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 408 KB
• Volume: 21
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200804439

✦ Synopsis

Abstract

Gold nanoparticles (AuNP) stabilized by the synthetic membrane‐like compound didodecyldimethylammonium bromide (DDAB) were used for the modification of graphite screen‐printed electrodes (SPE). Two different electrochemical approaches based on AuNP modified sensors for the detection of bioaffinity interactions were developed. As a model human cardiac myoglobin–complementary antimyoglobin antibody couple was taken. In the first method AuNP worked as an electrochemical sensing platform: the oxidation of the gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. In the second assay, AuNP served as effective electrocatalysts of Fe^3+^/Fe^2+^ electrode reaction of myoglobin. For both assays the same AuNP synthesis and electrodes modification procedures were used.