Electrochemical Investigation of Glucose Sensor Fabricated at Copper-Plated Screen-Printed Carbon Electrodes
✍ Scribed by Annamalai Senthil Kumar; Jyh-Myng Zen
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 253 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Copper-plated screen-printed carbon electrode (CuSPE) provides a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Glucose oxidase (GOD) is immobilized on the top of the CuSPE to form a glucose sensor. The interaction of copper oxide with GOD was found to be an important factor in the glucose detection. Preliminary investigation under hydrodynamic conditions showed a linear calibration plot up to 26.7 mM glucose with a slope and regression coefficient of 4.5 mA/mM and 0.9902, respectively. The Michaelis-Menten kinetics by nonlinear curve fitting yielded a K m value close to that in solution indicating the ideality and suitability of the present system. Classical mixed potential mechanism is for the first time applied to the enzyme-coated CuSPE to further understand the system.