Electrochemical Immunoassay of Human Chorionic Gonadotrophin Based on Its Immobilization in Gold Nanoparticles-Chitosan Membrane

✍ Scribed by Jin Chen; Feng Yan; Dan Du; Jie Wu; Huangxian Ju

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 407 KB
• Volume: 18
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200503440

✦ Synopsis

Abstract

A human chorionic gonadotrophin (hCG) doped gold nanoparticles–chitosan membrane was prepared for forming an immunoconjugate of horseradish peroxidase labeled hCG antibody and hCG on glassy carbon electrode. The nanoparticles provided a congenial environment of the adsorbed proteins. Thus, the immobilized HRP‐labeled immunoconjugate showed good enzymatic activity for the oxidation of o‐phenylenediamine by H~2~O~2~. With a competitive mechanism, an amperometric method for immunoassay of hCG up to 30 mIU mL^−1^ with a relatively low detection limit of 0.26 mIU mL^−1^ at 3__σ__ was developed. The hCG immunosensor showed good precision, high sensitivity, acceptable stability and reproducibility.