Abstract
Release of nitric oxide (NO) is of high importance for regulating endothelial cell functions during vasodilatation, vascular remodeling, and angiogenesis. Thus, a direct and reliable real‐time method for NO detection that takes into account time‐dependent variations of the NO concentration in the complex reaction within the diffusion zone above the cells is vital for obtaining information about the role of NO in intracellular endothelial signal transduction and its impact on the surrounding cells. In this study, the time course of vascular endothelial growth factor E (VEGF‐E) stimulated NO release from transformed human umbilical vein endothelial cells (T‐HUVEC) was investigated by means of metalloporphyrin‐based NO sensors employed in an electrochemical robotic system. The NO sensor was obtained by electrochemically induced deposition of Ni^II^ tetrakis(p‐nitrophenylporphyrin) on a 50‐μm diameter platinum disk electrode which was integrated, together with a 25‐μm diameter platinum disk, in a double‐barrel electrode arrangement. The second electrode was used as a guidance sensor for the automatic and highly reproducible positioning of the NO sensor at a known distance from a layer of adherently growing cells by using z‐approach curves in the negative feedback mode of scanning electrochemical microscopy (SECM). The electrochemical robotic system allows the fully automated detection of NO with high sensitivity and selectivity to be performed in real time within 96‐well microtiter plates. A functional cell assay was established to allow the standardized detection of NO released upon stimulation from T‐HUVEC with a sensor positioned at a known distance above the endothelial cells. The overall system was evaluated by automatic detection of NO release from T‐HUVEC upon stimulation with VEGF‐E after incubation with a variety of drugs that are known to act on different sites in the complex signal‐transduction pathway that finally invokes NO release.