Electrochemical enzyme immunoassay using sequential saturation technique in a 20-μl capillary: digoxin as a model analyte
✍ Scribed by Noriaki Kaneki; Yan Xu; Anu Kumari; H.Brian Halsall; William R. Heineman; Peter T. Kissinger
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 546 KB
- Volume
- 287
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Capillary enzyme immunoassay with flow-injection analysis for digoxin using the sequential saturation technique has been developed. Glass capillary tubes (10 cm x 0.53 mm i.d.) with immobilized digoxin antibody were used as the immunoassay reactor. The product of enzymatic reaction, 4-aminophenol, was detected amperometrically. The digoxin and the labeled digoxin binding reaction with the immobilized digoxin antibody were completed in 2 and 10 min, respectively. Digoxin was determined in a 2O-pl sample with a detection limit of 10 pg ml-' (200 fg or 260 attomoles) and a 3 orders of magnitude range.