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Electrochemical DNA Biosensors Based on Palladium Nanoparticles Combined with Carbon Nanotubes

✍ Scribed by Zhu Chang; Hao Fan; Kun Zhao; Miao Chen; Pingang He; Yuzhi Fang


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
200 KB
Volume
20
Category
Article
ISSN
1040-0397

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✦ Synopsis


Abstract

Palladium nanoparticles, in combination with multi‐walled carbon nanotubes (MWCNTs), were used to fabricate a sensitivity‐enhanced electrochemical DNA biosensor. MWCNTs and palladium nanoparticles were dispersed in Nafion, which were used to modify a glassy carbon electrode (GCE). Oligonucleotides with amino groups at the 5′ end were covalently linked onto carboxylic groups of MWCNTs on the electrode. The hybridization events were monitored by differential pulse voltammetry (DPV) measurement using methylene blue (MB) as an indicator. Due to the ability of carbon nanotubes to promote electron‐transfer and the high catalytic activities of palladium nanoparticles for electrochemical reaction of MB, the sensitivity of presented electrochemical DNA biosensors was remarkably improved. The detection limit of the method for target DNA was 1.2×10^−13^ M.


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