Electrochemical Dissolution of Silver Nanoparticles and Its Application in Metalloimmunoassay

✍ Scribed by Mateusz Szymanski; Anthony P. F. Turner; Robert Porter

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 448 KB
• Volume: 22
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200900275

✦ Synopsis

Abstract

This work demonstrates the use of silver nanoparticles as a simple electrochemical biolabel to induce 10^6^ signal enhancement. We propose a mechanism of measuring the silver nanoparticles on a specific screen‐printed planar carbon electrode, without the requirement for the harsh oxidant or toxic reagents described in prior‐art for gold sol methods. The possible measurement process was validated with orthogonal techniques such as UV/Vis spectroscopy, dynamic light scattering and by anodic stripping voltammetry (ASV). The findings were utilized to develop a novel electrochemical sandwich immunoassay where the analyte concentration is directly proportional to ASV oxidation peak of silver. This technique in the future is envisaged to form the foundation of a generic Point of Care platform. The assay was applied to cardiac marker: myoglobin with detection limit of 3 ng/mL.