Electrochemical Detection of Surface Hybridization of Oligodeoxynucleotides Bearing Anthraquinone Tags at Gold Electrodes

✍ Scribed by Vilmos Kertesz; Neil A. Whittemore; Gopal B. Inamati; Muthiah Manoharan; P. Dan Cook; David C. Baker; James Q. Chambers

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 146 KB
• Volume: 12
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/1521-4109(200008)12:12<889::aid-elan889>3.0.co;2-k

✦ Synopsis

Gold electrodes modi®ed with a DNA 20-mer via a thiol linkage were shown to ef®ciently capture the complementary 20-mer that was tagged with anthraquinone (AQ) reporter groups. Treatment of the electrodes with 2-mercaptoethanol was required for both ef®cient surface hybridization and for reversible electron transfer between the gold electrode and the tethered anthraquinone tag. In addition, slow thermal annealing through the melting temperature of the duplex increased the ef®ciency of the process. Electrodes that had been modi®ed with a ds-DNA bearing AQ tags gave slightly greater voltammetric signals, but after dissociation of the duplex were not as effective as the ss-DNA in capturing the complementary 20-mer from the buffer solutions.