Electrochemical desorption of self-assembled monolayers for engineering cellular tissues

✍ Scribed by Rina Inaba; Ali Khademhosseini; Hiroaki Suzuki; Junji Fukuda

• Book ID: 104003844
• Publisher: Elsevier Science
• Year: 2009
• Tongue: English
• Weight: 676 KB
• Volume: 30
• Category: Article
• ISSN: 0142-9612
• DOI: 10.1016/j.biomaterials.2009.03.045

✦ Synopsis

Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm 2 and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications.