Electroblotting of individual polypeptides from SDS/polyacrylamide gels for direct sequence analysis
✍ Scribed by Tomas BERGMAN; Hans JÖRNVALL
- Book ID
- 115125401
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 387 KB
- Volume
- 169
- Category
- Article
- ISSN
- 1432-1327
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📜 SIMILAR VOLUMES
A procedure for the carbohydrate analysis of glycoproteins electrotransferred to a polyvinylidene difluoride membrane is described. The glycoproteins (plant lectins, transferrin, and vitronectin) were first separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then el
Proteins which are electroblotted from native gels onto polyvinylidene difluoride (PVDF) membranes are suitable for detailed structural analysis. This method, in conjunction with limited proteolysis and N-terminal sequencing, has been used to study the molecular interactions between native protein m