Electroanalytical assay of human prekallikrein activation from whole blood dilutions

✍ Scribed by E. Corretge; J.M. Nigretto

• Book ID: 103067229
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 469 KB
• Volume: 32
• Category: Article
• ISSN: 0013-4686
• DOI: 10.1016/0013-4686(87)87045-7

✦ Synopsis

The extent of the stimulation of blood coagulation can be determined by assaying the generation of kallikrein, a serine protease. This enzyme is able to cleave specific and synthetic substrates designed for that purpose. Clinical assays of plasma dilutions are thus carried out by monitoring spectrophotometrically the release of chromogenic labels. Such methods however are not applicable to whole blood. Results given show that the kinetics of kallikrein generation can be determined electrochemically in whole blood. Data, obtained by amperometry from the oxidation of an electroactive label, compare to plasma by taking into account the haematocrit.