Isolation and primary culture of urothel
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Rahman, Z. ;Reedy, A. ;Heatfield, B.M.
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Article
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1987
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Springer
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English
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Trypsinization (WT) was employed to disaggregate urothelial cells from normal human urinary bladder mucosa for the preparation of primary cultures. Urinary bladders of two male adults both 25 years old were obtained autopsy 1-2 h after death. The mucosa was incubated in HBSS containing 0.25% trypsin