Electric field gradients and band sharpening in DNA gel electrophoresis
โ Scribed by Gary W. Slater; Dr. Jaan Noolandi
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 451 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Abstract Polyacrylamide gel electrophoresis (PAGE) is used frequently for isolation and purification of DNA fragments. In the present study, DNA fragments extracted from polyacrylamide gels showed significant band broadening in capillary electrophoresis (CE). A pHY300PLK (a shuttle vector functi
Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa