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Electric birefringence and dichroism of acridine orange and methylene blue complexes with polynucleotides

✍ Scribed by D. F. Bradley; N. C. stellwagen; C. T. O'konski; C. M. Paulson

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
426 KB
Volume
11
Category
Article
ISSN
0006-3525

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✦ Synopsis

Electro-optic measurements are reported for the polynucleotides poly A, poly U, poly G, and NaDNA, and their complexes with acridine orange (AO). Measurements were also made on the methylene blue (MB) complex with NaDNA. Poly U, poly G, and NaDNA complexes with A 0 as well as the NaDNA-MB complex were found to exhibit positive birefringence and perpendicular dichroism indicating the dye molecules are oriented with their long axes perpendicular to the applied electric field. The opposite was found for the A 0 complex with poly A, which showed positive birefringence and parallel dichroism, indicating that in this case the dye molecules are oriented with their long axes parallel to the field.

* We owe much to the late D. F. Bradley who collaborated in the planning and in the performance of this research during a brief stay in Berkeley, as well as in preparing preliminary drafts of this manuscript.

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