✦ LIBER ✦

Elastin repeat peptides as chemoattractants for bovine aortic endothelial cells

✍ Scribed by Marianna M. Long; Vickie J. King; Kari U. Prasad; Bruce A. Freeman; Dan W. Urry

Book ID: 102882022
Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 758 KB
Volume: 140
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041400316

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✦ Synopsis

Cultured bovine aortic endothelial cells migrate toward a concentration gradient of repeating elastin peptides, specifically the repeating nonamers Gly-Phe-Gly-Val-Cly-Ala-Gly-Val-Pro and Gly-Leu-Cly-Val-Gly-Ala-Gly-Val-Pro and the repeating hexamer Val-Cly-Val-Ala-Pro-Gly. Dose-response experiments demonstrate that the peak of activity occurs at 8 x 1 OP8 M for the nonapeptides and 1 x 1 0-8 M for the hexapeptide. Checkerboard assays establish that the movement is chernotaxis and not chemokinesis. Because of the concentration difference in the responsiveness between the nonapeptide and the hexapeptide, the cells can differentiate between the two types of repeats. The positive control for the chernotaxis studies was fibronectin.

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