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Elastin-like recombinamers as substrates for retinal pigment epithelial cell growth

✍ Scribed by Girish K. Srivastava; Laura Martín; Amar K. Singh; Ivan Fernandez-Bueno; Manuel J. Gayoso; Maria T. Garcia-Gutierrez; Alessandra Girotti; Matilde Alonso; José C. Rodríguez-Cabello; José C. Pastor

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
562 KB
Volume
97A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

The aim of this study is to investigate the use of elastin‐like recombinamers (ELRs) as a substrate that can maintain the growth, phenotype, and functional characteristics of retinal pigment epithelial (RPE) cells efficiently and as a suitable carrier for the transplantation of autologous RPE cells for treatment of age‐related macular degeneration (AMD). ELR films containing a bioactive sequence, RGD (ELR‐RGD), and one with no specific sequence (ELR‐IK) as control, were obtained by solvent‐casting onto glass and subsequent cross‐linking. ARPE19 cells were seeded on sterilized ELR films as well as on the control surfaces. Cells were analysed after 4, 24, 72, and 120 h to study cell adhesion, proliferation, cell viability, morphology, and specificity by staining with Trypan blue, DAPI, Rhodamin‐Phalloidin and RPE65, ZO‐1 antibodies and observing under fluorescence as well as electron microscope. ARPE19 cells seeded on both ELR films and controls were 100% viable and maintained their morphology and set of characteristics at the different time points studied. Cell proliferation on ELR‐RGD was significantly higher than that found on ELR‐IK at all time points, although it was less than the growth rate on polystyrene. ARPE19 cells grow well on ELR‐RGD maintaining their phenotype. These results should be extended to further studies with fresh human RPE cells and in vivo studies to determine whether this ELR‐RGD matrix could be used as a Bruch's membrane prosthesis and carrier for transplantation of RPE cells in patients suffering with AMD. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.

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