Einsatz der Phenol-Wasser-Extraktion zur Isolierung des gruppenspezifischen Polysaccharidkomplexes aus B-Streptokokken
✍ Scribed by M. Mehl; Dr. med. R. Starke; Claudia Möckel; W. Presber
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 514 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
The group specific polysaccharide of Group B streptococci was isolated either by means of phenol-water extraction or by Lancefield extraction procedures. Two groups of rabbits were immunized with these substances to induce polyclonal antibodies. The phenol-water extract induced antisera had a significantly higher titer against B streptococci than against A-, D- and G streptococci in the agglutination reaction. The B-specific titers in rabbits immunized with phenol-water extract were significantly higher than those obtained in the rabbits immunized with Lancefield extract. In counter immunoelectrophoresis, only the antisera prepared by immunization with phenol-water extract reacted with standard antigen from group B streptococci. The serological specificity of the phenol-water extract antigen isolated from B streptococci was confirmed by counterimmunoelectrophoresis. The antigen was a heterologous fraction with a molecular mass of 8 x 10(4)-2 x 10(6) Dalton.