Einsatz der Fermenter-Dialyse-Kultur zur Züchtung kapseltragender Bakterien mit klinischer Bedeutung

✍ Scribed by Dr. Roland Starke; Michael Mehl; Claudia Möckel; Roland Grunow; Thomas Montag; Rüdiger von Baehr; Gerhard Schmidt

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 421 KB
• Volume: 29
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620290316

✦ Synopsis

For mass cultivation of group B streptococci (B-III and B-variant streptococci), a fermenter dialysis culture technique is described and compared to conventional shaking culture and fermenter batch culture techniques. The influence of two kinds of cultivation media on the bacterial yield is demonstrated. The growth rate of the bacteria and the yield of the microbes is higher in modified POPE medium than that observed with Nährbouillon I. The type-specific polysaccharide of B-III-streptococci prepared by phenol-water extraction followed by gel-chromatography can be used as a screening antigen for the production of monoclonal antibodies against B-III-streptococci.