Einfluß einiger Faktoren in Abhängigkeit von den Methoden bei der Immobilisierung eines bakteriellen Enzympräparats mit Milchkoagulationswirkung
✍ Scribed by E. Dobreva
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 285 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
The influence of different factors (binding time, pH, temperature, and enzyme/carrier ratio) on the efficiency of immobilization of enzyme preparation with milk clotting activity from Bacillus mesentericw M strain by use of different carriers and methods of binding was investigated. It has been established that the dependence of immobilization from pH is most pronounced and is mainly influenced by the nature of the carrier. The binding time is of essential importance only by immobilization through adsorption. The temperature has not any influence on the efficiency of immobilization of the indicate preparation. Saturation of many of the used carriers by the different methods for immobilization was achieved a t enzymelcarrier ratio 1 : 10, with exception of the cases where glntaraldehyde was used as binding reagent. I n this case then the ratio was 1 : 4.