Eine Trennkammer für die Hochspannungselektrophorese nach dem Michl'- schen Prinzip

✍ Scribed by B. Kickhöfen; R. Warth

• Book ID: 104145469
• Publisher: Elsevier Science
• Year: 1968
• Tongue: English
• Weight: 324 KB
• Volume: 33
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98693-x

✦ Synopsis

NOTES

Corp.). The dinuclcotides had, been separated from a silkworm endonuclease digest of yeast RNA by DEAE-cellulose column chromatography with 7 IM uread, and then desalted ,, by ,, a ,DEAE-cellulose bicarbonate, method s. The demonophosphorylation brought about an increased'mobility.

and,a wider distribution of the nucleotides, and so improved the chromatogram greatly.

As far as is known, it has not been possible to separate dinucleosidic compounds of all the possible base.pairings by a simple method. The present proposed method is also applicable to either 5'-or'3'-terminal dinucleotides or dinucleoside triphosphates since they all are easily and quantitatively converted to dinucleoside monophosphates enzymatically, and so may be widely used in the structural and enzymological work of nucleic acids.

We wish to thank Drs. H. OKAZAKT and I<. ISHIBASHI, Sallkyo Co. Ltd., Tokyo for a generous gift of purified ribonuclease TV.