Eine partikelgebundene Transfer-Ribonucleinsäure aus Rattenleber

✍ Scribed by A. Hicklin; F. Leuthardt

• Book ID: 102250227
• Publisher: John Wiley and Sons
• Year: 1963
• Tongue: German
• Weight: 402 KB
• Volume: 46
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19630460407

✦ Synopsis

Abstract

Incubation of microsonics with the supernatant of pH−5‐enzymes is as active in incorporating amino‐acids into proteins (controlled by use of leucin‐[^14^C]) as incubation with the usual system, which is supplied with pH−5‐enzymes. Since the supernatant of pH−5‐enzymes does not contain any soluble ribonucleic acid (sRNA), all the le∼ciri‐[^14^C] found in nucleic acids must be hound to microsonial KNA. The behaviour of the isolated microsomal KNA on Sephadex G 75, and of the ribonuclease digest in paper electrophoresis, confirms our suggestion of the presence of an active sRNA fraction in microsomes and ribosomes.