Eine neue Methode zur Carboxyh�moglobinbestimmung in fl�ssigem und getrocknetem Blut mittels Fourier-Transform-Infrarot-Spektrometrie (FTIR)
✍ Scribed by Kijewski, H. ;Seefeld, K.P. ;P�hlmann, K.
- Publisher
- Springer-Verlag
- Year
- 1985
- Tongue
- English
- Weight
- 425 KB
- Volume
- 95
- Category
- Article
- ISSN
- 1437-1596
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✦ Synopsis
A new technique is presented for the fast quantitative determina- tion of carboxy-hemoglobin (CoHb) in liquid blood with the aid of Fourier transformation infrared spectrometry.
The bands at P = 1953 and 1969 (cm-1), which are characteristic for the CoHb complex, were measured without the blood being prepared. In liquid samples the band at 1953 cm -1 is prominent; in dry samples, however, it appears. In moist samples both bands--each to be coordinated with a different CoHb conformer--are observed at about the same intensity. Even with dried blood spots of less than 0.1 rag, quantitative determination of CoHb at = 1969 (cm -1) is possible. Systematic investigations of the thermic stability of the CoHb complex were performed.
The typical interference phenomena associated with UV spectrometry were not observed when the above-described method was employed nor are they to be expected.
Even under extreme measuring conditions, as for example when measuring transmission through lab-coat material, different CoHb contents could be determined in traces of dried blood.