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Egg white lysozyme purification with a chitin–silica-based affinity chromatographic matrix

✍ Scribed by Federico J. Wolman; Guillermo J. Copello; Andrea M. Mebert; Alexandra M. Targovnik; María V. Miranda; Agustín A. Navarro del Cañizo; Luis E. Díaz; Osvaldo Cascone

Book ID
105900321
Publisher
Springer
Year
2010
Tongue
English
Weight
368 KB
Volume
231
Category
Article
ISSN
0044-3026

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Efficient wheat germ agglutinin purifica
Efficient wheat germ agglutinin purification with a chitosan-based affinity chromatographic matrix
✍ María F. Baieli; Nicolás Urtasun; María V. Miranda; Osvaldo Cascone; Federico J. 📂 Article 📅 2012 🏛 John Wiley and Sons 🌐 English ⚖ 291 KB

## Abstract An efficient affinity chromatographic matrix based on chitosan for wheat germ agglutinin (WGA) purification was developed. The matrices assayed consisted of chitosan mini‐spheres cross‐linked with epichlorhydrin 45, 250 or 500 mM. The maximum adsorption capacity of pure WGA – calculated