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EGFP as a fusion partner for the expression and organic extraction of small polypeptides

✍ Scribed by Vitaly S Skosyrev; Natalja V Rudenko; Alexander V Yakhnin; Vasily E Zagranichny; Lubov I Popova; Mikhail V Zakharov; Andrey Yu Gorokhovatsky; Leonid M Vinokurov


Book ID
117702602
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
305 KB
Volume
27
Category
Article
ISSN
1046-5928

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✍ Stewart D. Nuttall; Mat J. M. Rousch; Robert A. Irving; Simon E. Hufton; Hennie πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 311 KB πŸ‘ 2 views

We have designed and engineered the human cytotoxic T-lymphocyte associated protein-4 (CTLA-4) variable (V-like) domain to produce a human-based protein scaffold for peptide display. First, to test whether the CTLA-4 CDR-like loops were permissive to loop replacement/insertion we substituted either