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Efforts Toward the Direct Experimental Characterization of Enzyme Microenvironments: Tyrosine100 in Dihydrofolate Reductase

✍ Scribed by Dan Groff; Megan C. Thielges; Susan Cellitti; Peter G. Schultz; Floyd E. Romesberg


Book ID
101539439
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
309 KB
Volume
121
Category
Article
ISSN
0044-8249

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Efforts Toward the Direct Experimental C
✍ Dan Groff; Megan C. Thielges; Susan Cellitti; Peter G. Schultz; Floyd E. Romesbe 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 309 KB

## Abstract **State secrets**: Site‐specific deuteration and FTIR studies reveal that Tyr100 in dihydrofolate reductase plays an important role in catalysis, with a strong electrostatic coupling occuring between Tyr100 and the charge that develops in the hydride‐transfer transition state (see pictu