Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells
✍ Scribed by André Strauss; Francis Bitsch; Gabriele Fendrich; Patrick Graff; René Knecht; Bernd Meyhack; Wolfgang Jahnke
- Book ID
- 106401181
- Publisher
- Springer Netherlands
- Year
- 2005
- Tongue
- English
- Weight
- 369 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0925-2738
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## Abstract Recombinant baculoviruses were constructed to express cDNAs encoding two distinct subtypes of human cAMP‐specific phosphodiesterase (hPDE4A and hPDE4B). Infection of Spodoptera frugiperda insect cells with the appropriate recombinant baculoviruses resulted in high level production of bi
The baculovirus/insect cell system (Autographa and function of proteins. One post-translational modicalifornica multiple nuclear polyhedrosis virus/Spodopfication which is currently being studied is the attachtera frugiperda Sf9 cells) was used to express the GPIment of preassembled glycosylphosphat