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Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation

โœ Scribed by Lenka Pribylova; Hana Sychrova


Book ID
117360222
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
86 KB
Volume
55
Category
Article
ISSN
0167-7012

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Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f