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Efficient transformation of filamentous fungusPleurotus ostreatususing single-strand carrier DNA

โœ Scribed by Toshikazu Irie; Yoichi Honda; Takashi Watanabe; Masaaki Kuwahara

Book ID
105953947
Publisher
Springer
Year
2001
Tongue
English
Weight
26 KB
Volume
55
Category
Article
ISSN
1432-0614

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A method, using LiAc to yield competent cells, is described that increased the efficiency of genetic transformation of intact cells of Saccharomyces cerevisiae to more than 1 X 105 transformants per microgram of vector DNA and to 1.5 % transformants per viable cell. The use of single stranded, or he