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Efficient transfer of chromosome-based DNA constructs into mammalian cells

โœ Scribed by Volker Oberle; Gary de Jong; Jan I. Drayer; Dick Hoekstra

Book ID
113485263
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
270 KB
Volume
1676
Category
Article
ISSN
0167-4781

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Electroporation, the reversible breakdown of cell membranes caused by a high-voltage discharge, is a rapid, simple, and efficient method for introducing DNA into mammalian cells. An instrument for electroporation which permits the high-voltage discharge waveform to be varied with respect to rise tim