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Efficient synthesis of γ-methyl-capped guanosine 5′-triphosphate as a 5′-terminal unique structure of U6 RNA via a new triphosphate bond formation involving activation of methyl phosphorimidazolidate using ZnCl2 as a catalyst in DMF under anhydrous conditions

✍ Scribed by Michinori Kadokura; Takeshi Wada; Chihiro Urashima; Mitsuo Sekine


Book ID
104258315
Publisher
Elsevier Science
Year
1997
Tongue
French
Weight
270 KB
Volume
38
Category
Article
ISSN
0040-4039

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✦ Synopsis


p1-Methyl P3-(5'-guanosyl) triphosphate (CH3pppG), which exists as a unique structure at the 5'-terminal region of U6 RNA, was synthesized in an excellent yield by methyl phosphorimidazolidate (2c) with the bis(tetrabutylammonium) salt of GDP in the presence of ZnCI2 as an effective catalyst in dry DMF.