Efficient sequence analysis of the six gene products (7-74 kDA) from the escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry
✍ Scribed by Neil L. Kelleher; Sean V. Taylor; David Grannis; Cynthia Kinsland; Hsiu-Ju Chiu; Tadhg P. Begley; Fred W. Mclafferty
- Book ID
- 105356553
- Publisher
- Cold Spring Harbor Laboratory Press
- Year
- 1998
- Tongue
- English
- Weight
- 638 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0961-8368
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✦ Synopsis
Abstract
The 10^5^resolving power and MS/MS capabilities of Fourier‐transform mass spectrometry provide electrospray ionization mass spectra containing > 100 molecular and fragment ion mass values of high accuracy. Applying these spectra to the detection and localization of errors and modifications in the DNA‐derived sequences of proteins is illustrated with the thiCEFSGH thiamin biosynthesis operon from Escherichia coli. Direct fragmentation of the multiply‐charged intact protein ions produces large fragment ions covering the entire sequence; further dissociation of these fragment ions provides information on their sequences. For ThiE (23 kDa), the entire sequence was verified in a single spectrum with an accurate (0.3 Da) molecular weight (M~r~) value, with confirmation from MS/MS fragment masses. Those for ThiH (46 kDa) showed that the M~r~ value (1 Da error) represented the protein without the start Met residue. For ThiF (27 kDa), MS/MS localized a sequence discrepancy to a 34 residue peptide. The first 107 residues of ThiC (74 kDa) were shown to be correct, with C‐terminal heterogeneity indicated. For ThiG (predicted M~r~ = 34 kDa), ESI/FTMS showed two components of 7,310.74 (ThiS) and 26,896.5 Da (ThiG); MS/MS uncovered three reading frame errors and a stop codon for the first protein. MS/MS ions are consistent with 68 fragments predicted by the corrected ThiS/ThiG DNA sequences.