Efficient screening a high glutathione-content mutant of Saccharomyces cerevisiae by flow cytometry
โ Scribed by Zheng Wang; Liyang Zhang; Tianwei Tan
- Book ID
- 108254760
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 530 KB
- Volume
- 45
- Category
- Article
- ISSN
- 1873-3298
No coin nor oath required. For personal study only.
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## Abstract The production of enriched fructose syrups and ethanol from a synthetic medium with high sucrose concentrations was studied in a batch process using __Saccharomyces cerevisiae__ ATCC 36858. The results showed that the fructose yield was above 92% of theoretical values in synthetic media
Background: Analysis of the DNA cell cycle and glutathione content cannot be performed on viable cells, because the fluorescence emissions of the DNA-specific probe Hoechst 33342 and the glutathione-specific probe monobromobimane overlap completely. We decided to explore whether the emissions could