𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Efficient regeneration ofBrassica napusL. hypocotyls and genetic transformation byAgrobacterium tumefaciens

✍ Scribed by P. Jonoubi; A. Mousavi; A. Majd; A.H. Salmanian; M. Jalali Javaran; J. Daneshian

Publisher
Springer Netherlands
Year
2005
Tongue
English
Weight
769 KB
Volume
49
Category
Article
ISSN
0006-3134

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Efficient regeneration ofBrassica olerac
Efficient regeneration ofBrassica oleraceahypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake
✍ Arundhati Mukhopadhyay; Reinhard Töpfer; Akshay K. Pradhan; Yaspal S. Sodhi; Han 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 850 KB

Efficient regeneration (80%) and high frequency genetic transformation (10-33%) were achieved by culturing protoplasts isolated from hypocotyl tissues of six day old Brassica oleracea seedlings and by subjecting these protoplasts to PEG mediated direct plasmid uptake. Three different plasmid vectors