Efficient Purification of Recombinant Proteins Using Hydrophobins as Tags in Surfactant-Based Two-Phase Systems †

Purification of recombinant and human ap

Purification of recombinant and human apolipoprotein A-1 using surfactant micelles in aqueous two-phase systems: Recycling of thermoseparating polymer and surfactant with temperature-induced phase separation

✍ Josefine Persson; Lena Nyström; Hans Ageland; Folke Tjerneld 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 299 KB 👁 2 views

An effective system has been developed for purification of apolipoprotein A-1 from Escherichia coli fermentation solution and human plasma using aqueous two-phase extraction and thermal-phase separation. The system included non-ionic surfactants (Triton or Tween) and as top phase-forming polymer a r