Efficient purification and morphology characterization of paclitaxel from cell cultures of Taxus chinensis

✍ Scribed by Sang-Hyun Pyo; Moon-Suk Kim; Jin-Suk Cho; Bong-Kyu Song; Byung-Hee Han; Ho-Joon Choi

• Publisher: Wiley (John Wiley & Sons)
• Year: 2004
• Tongue: English
• Weight: 220 KB
• Volume: 79
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.1111

✦ Synopsis

Abstract

Paclitaxel was purified from cell cultures of Taxus chinensis by a combination of extraction, low‐pressure chromatography, precipitation and HPLC. A crude extract (purity 6.9%) was obtained by methanol extraction of plant cell cultures, followed by liquid–liquid extraction using dichloromethane. The extract was purified to greater than 32% with a 97% step yield by low‐pressure chromatography. After acetone/pentane precipitation, the resulting purity and step yield were 75.8% and 97.4%, respectively. High performance liquid chromatography steps, which were composed of an HPLC step with column‐packed ODS and an HPLC step with column‐packed silica, were applied to give over 99.5% purity with high yield. Amorphous paclitaxel with a fine particle size, which has a solubility advantage compared with the stable crystalline form, was obtained by dissolving in dichloromethane, followed by spray‐drying. Copyright © 2004 Society of Chemical Industry