Efficient plant regeneration from long-term callus cultures of rice by spermidine

✍ Scribed by S. Bajaj; M. V. Rajam

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 433 KB
• Volume: 14
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232654

✦ Synopsis

A significant reduction in regeneration potential with increasing age (upto 12months) in rice (Oryza sativa L. cv.TN-1) embryogenic callus cultures was observed. Spermidine, while having an inhibitory effect on plant regeneration in fresh callus cultures, promoted morphogenesis in long-term callus cultures. A massive accumulation of polyamines, particularly putrescine (5-fold) was observed in 12 month old cultures resulting in a change of putrescine /spermidine ratio, which seems to be important for maintaining the morphogenetic response. Application of exogenous spermidine to 12 month old cultures showed increased levels of polyamines and restored the putrescine/spermidine ratio comparable to that found in freshly induced cultures, concomitantly, promoting the plant regeneration via somatic embryogenesis in long-term rice callus cultures.