Efficient methods for obtaining phase-sensitive gradient-enhanced HMQC spectra

✍ Scribed by Alfred Ross; Michael Czisch; Christian Cieslar; Tad A. Holak

• Publisher: Springer Netherlands
• Year: 1993
• Tongue: English
• Weight: 524 KB
• Volume: 3
• Category: Article
• ISSN: 0925-2738
• DOI: 10.1007/bf00178263

✦ Synopsis

Three improved versions of the gradient-enhanced HMQC experiment are presented which yield phasesensitive spectra with increased sensitivity compared to the recently described field-gradient HMQC schemes. The first method uses a complex linear back-prediction in order to generate the FIDs at the tl = 0. With this approach, refocusing pulses on the heteronucleus are not necessary. The sequence is especially useful for larger proteins with short relaxation times for the coherences that evolve during tl. In the other two methods lower and shorter gradient pulses or asymmetric gradients are used to optimize sensitivity.