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Efficient Lipid-Mediated Transfection of DNA into Primary Rat Hepatocytes

✍ Scribed by Sheri L. Holmen, Matt W. Vanbrocklin, Robert R. Eversole, Susan R. Stapleton and Leonard C. Ginsberg


Book ID
125624544
Publisher
Society for In Vitro Biology
Year
1995
Tongue
English
Weight
624 KB
Volume
31
Category
Article
ISSN
1475-2689

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## Abstract We describe the use of a non‐viral, polyethylenimine‐based vector to transfect rat hepatocytes preserved under hypothermic storage. DNA sequences encoding __Escherichia coli__ β‐galactosidase and pea ferredoxin‐NADP(H) oxidoreductase (FNR), cloned into plasmids pCH110 and pKM4 respectiv