✦ LIBER ✦
Efficient Immortalization by SV40 T DNA of Skin Fibroblasts From Patients With Wilms' Tumor Associated With Chromosome 11p Deletion
✍ Scribed by Yoshio Kano; John B. Little
- Book ID
- 102945346
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 667 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0899-1987
No coin nor oath required. For personal study only.
✦ Synopsis
We report that transfection with a plasmid containing the N 4 0 early region (T) leads to a very high frequency of immortalization i n t w o different strains of human diploid fibroblasts, each with a partial deletion of the short arm of chromosome 1 1. Immortalization often occurred without a recognizable "crisis" phase. Preimmortal cells showed a high frequency of spontaneous mutations and chromosomal aberrations. These results suggest that a gene involved in the control of senescence of human cells in vitro may be associated with this chromosome.